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Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

Catalog # 10112-GP | R&D Systems, Inc. a Bio-Techne Brand
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10112-GP-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Lgr4/GPR48 protein
Cynomolgus Monkey Lgr4
(Ala25-Thr544)
Accession # NP_001252594
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

84 kDa

SDS-PAGE

100-111 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human R-Spondin 3 Recombinant Human R‑Spondin 3 (Catalog # 3500-RS/CF) is coated at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera binds with an ED50 of 6-36 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein Binding Activity

When Recombinant Human R-Spondin 3 Recombinant Human R-Spondin 3 (Catalog # 3500-RS/CF) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera (Catalog # 10112-GP) binds with an ED50 of 6-36 ng/mL.
Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Lgr4/GPR48 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-111 kDa and 200-220 kDa, respectively.

Formulation, Preparation and Storage

10112-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr4/GPR48

Lgr4 (leucine‑rich repeat GPR 4), also called GPR48 (G‑protein‑coupled receptor 48), is a seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (1‑6). Lgr4 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors (5). It acts at least in part by enhancing Wnt‑dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta ‑catenin (3, 4). Cynomolgus Monkey Lgr4 cDNA encodes 951 amino acids (aa), including a long N‑terminal extracellular domain with multiple LRR domains that may mediate ligand interaction. The LRR‑containing ECD of Cynomolgus Monkey Lgr4 shares 99% aa sequence identity with human Lgr4. Lgr4 is widely expressed in both embryo and adult. Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). In rodents, embryonic and adult expression includes liver, kidney, adrenals, bone/cartilage, and heart (2, 7‑9). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis (9). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling (5, 6). Lgr4 may be over‑expressed in carcinomas and may promote invasiveness and metastasis by down‑regulating p27Kip1 expression (10).

References

  1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
  2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
  3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
  4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.
  5. de Lau, W. et al. (2011) Nature 476:293.
  6. Ruffner, H. et al. (2012) PLoS ONE 7:e40975.
  7. Van Schoore, G. et al. (2005) Histochem. Cell Biol. 124:35.
  8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:2241.
  9. Song, H. et al. (2008) J. Biol. Chem. 283:36687.
  10. Gao, Y. et al. (2006) Cancer Res. 66:11623.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 4

Alternate Names

GPR48

Entrez Gene IDs

55366 (Human); 107515 (Mouse); 286994 (Rat); 102119503 (Cynomolgus Monkey)

Gene Symbol

LGR4

UniProt

Product Documents for Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Lgr4/GPR48 Fc Chimera Protein, CF

For research use only

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