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Recombinant Cotton Rat IL-13 Protein

Bio-Techne includes R&D Systems | Catalog # 6940-CL

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Carrier Free
6940-CL-025/CF

Catalog #
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With Carrier
6940-CL-025

Key Product Details

Source

CHO

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cotton rat IL-13 protein
Met1-His133, with a C-terminal 5-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly21

Predicted Molecular Mass

13.1 kDa

SDS-PAGE

15-30 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 3 ‑ 18 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6940-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6940-CL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13

IL‑13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1 ‑ 4). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha‑helix configuration (5, 6). Mature cotton rat IL‑13 shares 59%, 74%, and 72% amino acid sequence identity with human, mouse, and rat IL‑13, respectively. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (7, 8). IL‑13 has diverse activities on numerous cell types (9). On macrophages, IL‑13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL‑13 induces immunoglobulin class switching to IgE, up‑regulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL‑13 up‑regulates IL‑6 while down‑regulating IL‑1 and TNF‑ alpha production by fibroblasts and endothelial cells. IL‑13 binds with low affinity to IL‑13 R alpha1, triggering IL‑13 R alpha1 association with IL‑4 R alpha. This high affinity receptor complex also functions as the type 2 IL‑4 receptor complex (10, 11). Additionally, IL‑13 binds with high affinity to IL‑13 R alpha2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (12 ‑ 15). IL‑13 R alpha2 regulates the bioavailability of both IL‑13 and IL‑4 and is overexpressed in glioma and several bronchial pathologies (11, 16, 17). Compared to wild type IL‑13, the atopy‑associated R110Q variant of IL‑13 elicits increased responsiveness from eosinophils that express low levels of IL‑13 R alpha2 (18).

References

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. 90:3735.
  4. Blanco, J.C.G. et al. (2004) J. Interferon Cytokine Res. 24:21.
  5. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  6. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  7. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  8. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  9. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  10. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  11. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  12. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  13. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  14. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  15. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  16. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  17. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  18. Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

UniProt

Additional IL-13 Products

Product Documents for Recombinant Cotton Rat IL-13 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cotton Rat IL-13 Protein

For research use only

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