Recombinant Canine VEGF Protein

Name: Recombinant Canine VEGF Protein
Brand: R&D Systems
SKU: 1603-CV
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1603-CV

Citations (5)

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Key Product Details

Source:	E. coli
Accession #:	AAD29682
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Canine VEGF Protein

Source	E. coli-derived canine VEGF protein Pro28-Arg190
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Pro28
Predicted Molecular Mass	19 kDa (monomer)
Activity	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 1.5-7.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1603-CV

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1603-CV/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF₁₈₈, VEGF₁₈₂, and VEGF₁₆₄, have been identified in canine. Canine VEGF₁₆₄ shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co-receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1 - 7).

Item	Value
References	JingJing, L. et al. (2000) Mol. Cell Biochem. 214:23. Scheidegger, P. et al. (1999) Biol. Chem. 380:1449. Thurston, G. (2002) J. Anat. 200:575. Zachary, I. and Gliki, G. (2001) Cardiovasc. Res. 49:568. Chevalier, S. (2002) Mol. Cell Endocrinol.189:169. Robinson, C.J. and Stringer, S.E. (2001) J. Cell. Sci. 114:853. Wang, L. et al. (2003) J. Biol. Chem. 278:48848.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Recombinant Canine VEGF Protein (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Canine VEGF Protein include:

Species: Canine
Sample Types: Recombinant Protein
Applications: ELISA

LM Muellerlei et al. (2019-01-01), In-vitro binding analysis of anti-human vascular endothelial growth factor antibodies bevacizumab and aflibercept with canine, feline, and equine vascular endothelial growth factor Res. Vet. Sci., 124(0):233-238.
PMID: 30933891
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Ozelo M et al. (2014-01-01), Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response. Blood, 123(26):4045-53.
PMID: 24829206
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Rebuzzi L et al. (2007-01-01), Detection of vascular endothelial growth factor (VEGF) and VEGF receptors Flt-1 and KDR in canine mastocytoma cells. Vet. Immunol. Immunopathol., 115(3):320-33.
PMID: 17196258
Species: Canine
Sample Types: Plasma
Applications: ELISA (Capture)

Kamstock D et al. (2007-01-01), Evaluation of a xenogeneic VEGF vaccine in dogs with soft tissue sarcoma. Cancer Immunol. Immunother., 56(8):1299-309.
PMID: 17502972
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Lin TY et al. (2006-01-01), A functional comparison of canine and murine bone marrow derived cultured mast cells. Vet. Immunol. Immunopathol., 114(3):320-34.
PMID: 17027994

Recombinant Canine VEGF Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Canine VEGF Protein

Formulation, Preparation and Storage

Carrier Free

1603-CV

Carrier

1603-CV/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Canine VEGF Protein (5)

Product Documents for Recombinant Canine VEGF Protein

Product Datasheets

COA

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