I was looking at the recombinant Protein G (NBP2-34962) and wanted to confirm that it does not contain a His tag

Name: Recombinant Protein G Cys (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-34962

I believe this protein does not have a histidine tag but it does have a CYS at the N-term.

Recombinant Protein G Cys (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34962

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

Cys (N-Term)

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Protein G Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 201 amino acids corresponding to Protein G with a Cys on the N-terminus. However it migrates with an apparent molecular mass of 40 kDa in SDS-PAGE Source: E. coli

Uniprot ID: P19909

Amino Acid Sequence: CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Protein G as determined by LAL method.

Predicted Molecular Mass

21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein G is a genetically engineered protein containing 3 IgG-binding regions of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein G binds to various human, mouse and rat IgG subclasses. It also binds to total IgG from cow, goat, sheep, house and rabbit.

Specificity

Protein G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and rabbit.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-34962

Formulation	Lyophilized from additive free solution.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Dissolve in distilled water or saline.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Protein G

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

Alternate Names

IgG binding protein G Immunoglobulin G binding protein G [Precursor] spg

Additional Protein G Products

Product Documents for Recombinant Protein G Cys (N-Term) Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Protein G Cys (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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