ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
R&D Systems, part of Bio-Techne | Catalog # PRD1845
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD1845 has been discontinued.
View all Sonic Hedgehog/Shh products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Product Specifications
Source
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met
Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
20 kDa
SDS-PAGE
22 kDa, reducing conditions
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.
The ED50 for this effect is typically 0.1-0.4 μg/mL.
The ED50 for this effect is typically 0.1-0.4 μg/mL.
Scientific Data Images for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein Bioactivity
ProDots®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 0.1-0.4 μg/mL.
ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 22 kDa.Formulation, Preparation and Storage
PRD1845
| Reconstitution | For a stock solution, reconstitute at 100 µg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thawcycles. • 6 months from date of receipt at room temperatureas supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions afterreconstitution. |
Background: Sonic Hedgehog/Shh
Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
Product Specific Notices for ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein
726244.0
For research use only
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