ProDots Recombinant Human R-Spondin 1 Protein
R&D Systems, part of Bio-Techne | Catalog # PRD4645
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD4645 has been discontinued.
View all R-Spondin 1 products.
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Arg31 - Ala263
Arg31 - Ala263
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser21 & Arg31
Predicted Molecular Mass
25.6 kDa
SDS-PAGE
39 kDa, reducing conditions
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The ED50 for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
The ED50 for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Scientific Data Images for ProDots Recombinant Human R-Spondin 1 Protein
ProDots Recombinant Human R-Spondin 1 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human R-Spondin 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 39 kDa.
ProDots Recombinant Human R-Spondin 1 Protein Bioactivity
ProDots®Recombinant Human R-Spondin 1, in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay.Formulation, Preparation and Storage
PRD4645
| Reconstitution | For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll ProDot® directly into cell culture medium. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Background: R-Spondin 1
Long Name
Roof Plate-specific Spondin 1
Alternate Names
Cristin 3, CRISTIN3, HRspo1, RSPO, RSPO1, RSpondin 1
Gene Symbol
RSPO1
UniProt
Additional R-Spondin 1 Products
Product Documents for ProDots Recombinant Human R-Spondin 1 Protein
Product Specific Notices for ProDots Recombinant Human R-Spondin 1 Protein
727085.0
For research use only
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