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Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06721

Novus Biologicals, part of Bio-Techne
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NBP3-06721

Key Product Details

Source

E. coli

Tag

Strep (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Flow Cytometry

Product Specifications

Description

A recombinant protein with a N-Terminal Strep-tag corresponding to 180 amino acids of Malus domestica 1.0101

Source: E.coli

Accession Numbers: XP_008390603.1

Store product aliquots at -20C to -70C. Use stored aliquots within 6 months.

Purity

>95%, by HPLC

Predicted Molecular Mass

19.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein

Flow Cytometry: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721]

Flow Cytometry: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721]

Flow Cytometry: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721] - Flow cytometry dot-plot staining pattern of Malus domestica 1.0101 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (NB100-77913) FITC and anti-human CD203c PE antibodies.
ELISA: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721]

ELISA: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721]

ELISA: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721] - ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen Malus domestica 1.0101 and allergen-specific human plasma IgG4 antibodies of Malus domestica positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Malus domestica 1.0101.
SDS-PAGE: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721]

SDS-PAGE: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721]

SDS-Page: Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein [NBP3-06721] - Recombinant allergen Malus domestica 1.0101 purity verification. 5 ug of Malus domestica 1.0101 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Formulation, Preparation and Storage

NBP3-06721
Preparation Method The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Formulation 100 mM Tris, 150 mM NaCl, pH 8.0
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution Reconstitute with sterile deionized water with or without azide.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Malus domestica 1.0101

Alternate Names

rMal d 1

Additional Malus domestica 1.0101 Products

Product Documents for Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Plant Malus domestica 1.0101 Strep (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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