Recombinant Luciferase (firefly) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18743
![Recombinant Luciferase (Firefly) Protein [NBP3-18743]—](https://resources.bio-techne.com/images/products/Recombinant-Luciferase-firefly-Protein-N-A-NBP3-18743-img0003.jpg "Recombinant Luciferase (Firefly) Protein [NBP3-18743]—")
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Functional Assay, SDS-PAGE
Product Specifications
Description
Luciferase from Photinus pyralis (firefly) recombinant, expressed in Escherichia coli, lyophilized powder, >= 10 x 10^10 units/mg protein
Purity
>98%, by SDS-PAGE
Predicted Molecular Mass
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Application Notes
Application: diagnostic assay manufacturing
Specific Activity: >= 10 x 10^10 light units/mg protein
Unit definition: One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25C over a 10 s period, measured in 100 ml assay mixture containing 40 pmole ATP and 15 nmole luciferin in Tris-glycine buffer, pH 7.6, using a GloMax(R) 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 uCi of 14C in a PPO/POPOP cocktail
Specific Activity: >= 10 x 10^10 light units/mg protein
Unit definition: One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25C over a 10 s period, measured in 100 ml assay mixture containing 40 pmole ATP and 15 nmole luciferin in Tris-glycine buffer, pH 7.6, using a GloMax(R) 20/20 Luminometer.
Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 uCi of 14C in a PPO/POPOP cocktail
Protein / Peptide Type
Full Length Recombinant Protein
Scientific Data Images for Recombinant Luciferase (firefly) Protein
![SDS-PAGE: Recombinant Luciferase (firefly) Protein [NBP3-18743]](https://resources.bio-techne.com/images/products/Recombinant-Luciferase-firefly-Protein-SDS-Page-NBP3-18743-img0001.jpg "SDS-PAGE: Recombinant Luciferase (firefly) Protein [NBP3-18743]")
SDS-PAGE: Recombinant Luciferase (firefly) Protein [NBP3-18743]
SDS-Page: Recombinant Luciferase (firefly) Protein [NBP3-18743] - 10% SDS-PAGE analysis![Functional: Recombinant Luciferase (firefly) Protein [NBP3-18743]](https://resources.bio-techne.com/images/products/Recombinant-Luciferase-firefly-Protein-Functional-NBP3-18743-img0002.jpg "Functional: Recombinant Luciferase (firefly) Protein [NBP3-18743]")
Functional: Recombinant Luciferase (firefly) Protein [NBP3-18743]
Functional: Recombinant Luciferase (firefly) Protein [NBP3-18743] - An ATP dose response curve was performed in 50 ul reaction cocktails containing decreasing amounts of ATP, 0.2 ug Luciferase, and 15 nmol luciferin in a Tris-glycine buffer, pH 7.6 supplemented with 0.5 mM EDTA, 5 mM MgSO4 and 0.1% BSA. Luminescence was measured at 22C for 10 s in quadruplicates, using a GloMax 20/20 Luminometer.Formulation, Preparation and Storage
NBP3-18743
| Preparation Method | Luciferase from Photinus pyralis (firefly) recombinant, expressed in Escherichia coli |
| Formulation | Lyophilized powder containing HEPES, pH 7.5, NaCl, MgCl2, EDTA, DTT and a carbohydrate stabilizer. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | To obtain maximal solubility it is important to reconstitute the enzyme at a high salt concentration, such as 1 M Tris buffer with any counter ion at pH 7-8. Can be prepared at a concentration of up to 5 mg protein/ml. Do not vortex and avoid agitation. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 degrees C. Avoid freeze/thaw cycles. |
Background: Luciferase (firefly)
The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.
References
1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9
2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005
3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b
4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036
Alternate Names
firefly luciferase
Additional Luciferase (firefly) Products
Product Documents for Recombinant Luciferase (firefly) Protein
Product Specific Notices for Recombinant Luciferase (firefly) Protein
Store the product at -20C. After reconstitution, the enzyme solutions can kept at 4-8C for up to 2 days or frozen in working aliquots at -20C for at least one month. Repeated freezing and thawing is not recommended. Do not store in a frost-free freezer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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