Recombinant Porcine Interleukin-1 receptor antagonist protein Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23517
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to Interleukin-1 receptor antagonist protein Source: E. coli
Uniprot ID: Q29056
Amino Acid Sequence: HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied..
- 1 month, 2 to 8 °C under sterile conditions after reconstitution..
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of IL-1 RAcP/IL-1 R3 as determined by LAL method.
Predicted Molecular Mass
17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IL1RAP Protein fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 10^4 IU/mg in the presence of 75 pg/ml rPoIL-1alpha.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP3-23517
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4, 1 mM DTT. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Interleukin-1 receptor antagonist protein
Alternate Names
IL1 inhibitor, IL-1ra, IL-1RN, interleukin-1 receptor antagonist protein, IRAP, IRAP1
Gene Symbol
IL1RN
Additional Interleukin-1 receptor antagonist protein Products
Product Documents for Recombinant Porcine Interleukin-1 receptor antagonist protein Protein
Product Specific Notices for Recombinant Porcine Interleukin-1 receptor antagonist protein Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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