Human IgM Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97056
Key Product Details
Source
Human serum
Conjugate
Unconjugated
Applications
Immunohistochemistry, Western Blot, ELISA, SDS-PAGE
Product Specifications
Description
Native Human Human IgM
Source: Human serum (Accession #:CAA00084.1)
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Source: Human serum (Accession #:CAA00084.1)
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Purity
Multi-step
Applications
Immunohistochemistry (1:10 - 1:500)
Western Blot (1:100 - 1:2000)
ELISA (1:100 - 1:2000)
Western Blot (1:100 - 1:2000)
ELISA (1:100 - 1:2000)
Application Notes
This product has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Protein / Peptide Type
Native Protein
Scientific Data Images for Human IgM Native Protein
![SDS-PAGE: Human IgM Native Protein [NBP1-97056]](https://resources.bio-techne.com/images/products/Human-IgM-Native-Protein-SDS-Page-NBP1-97056-img0001.jpg "SDS-PAGE: Human IgM Native Protein [NBP1-97056]")
SDS-PAGE: Human IgM Native Protein [NBP1-97056]
SDS-Page: Human IgM Native Protein [NBP1-97056] - IgM Whole Molecule. Lane 1: Human IgM, Reduced. M: Opal Pre-stained Marker. Lane 2: Human IgM, Non-Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.Formulation, Preparation and Storage
NBP1-97056
| Preparation Method | This product was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Human IgM (myeloma) whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgM (u chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross-reactivity will occur with anti-Human IgG. |
| Formulation | 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 |
| Preservative | 0.1% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles. |
Background: Human IgM
Alternate Names
AGM1, MU, VH
Additional Human IgM Products
Product Documents for Human IgM Native Protein
Product Specific Notices for Human IgM Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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