Centaurin beta 2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29037

Novus Biologicals, part of Bio-Techne
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NBP3-29037
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Centaurin beta 2 Antibodies »

Product Specifications

Immunogen

Between 728 and 778

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Centaurin beta 2 Antibody - BSA Free

Centaurin beta 2 Antibody

Immunoprecipitation:Centaurin beta 2 AntibodyNBP3-29037] -

Immunoprecipitation:Centaurin beta 2 AntibodyNBP3-29037] -Detection of human ACAP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACAP2 antibody NBP3-29037 (lot NBP3-29037-1) used for IP at 6 µg per reaction. ACAP2 was also immunoprecipitated by rabbit anti-ACAP2 antibody. For blotting immunoprecipitated ACAP2, NBP3-29037 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Centaurin beta 2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Centaurin beta 2

Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.

Alternate Names

Arf GAP with coiled coil, ANK repeat and PH domains 2, ArfGAP with coiled-coil, ankyrin repeat and PH domains 2, centaurin, beta 2, Centaurin-beta-2, CENTB2centaurin-beta-2, CNT-B2, KIAA0041arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Gene Symbol

ACAP2

Additional Centaurin beta 2 Products

Product Documents for Centaurin beta 2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Centaurin beta 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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