Recombinant Human ACAT2 His Protein

Name: Recombinant Human ACAT2 His Protein
Brand: Novus Biologicals
SKU: NBP1-45287

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45287

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ACAT2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-397 of Human ACAT2

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

45.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ACAT2 His Protein

SDS-PAGE: Recombinant Human ACAT2 His Protein [NBP1-45287]

SDS-Page: Recombinant Human ACAT2 Protein [NBP1-45287] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45287

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAT2

ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Acetyl-CoA acetyltransferase, cytosolic

Alternate Names

ACTL, EC 2.3.1.9

Gene Symbol

ACAT2

Additional ACAT2 Products

Product Documents for Recombinant Human ACAT2 His Protein

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COA

Product Specific Notices for Recombinant Human ACAT2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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