MTT Cell Proliferation Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54883

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP2-54883 has been discontinued. View all MTT Cell Proliferation products.

Key Product Details

Sample Type & Volume Required Per Well

Adherent and suspension cells, Proliferating and non-proliferating cells

View all MTT Cell Proliferation Activity Assays »

Product Specifications

Kit Type

Assay Kit (Colorimetric)

Reactivity

Human, Mouse, Rat, Mammal

Description

Fast and sensitive MTT based assay to measure cell proliferation in response to growth factors, cytokines, mitogens and nutrients.
    Detection method: Absorbance at 590 nm. Applications: Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators that inhibit cell growth.


This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab211091)

Kit Contents for MTT Cell Proliferation Assay Kit (Colorimetric)

  • MTT Reagent
  • MTT Solvent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: MTT Cell Proliferation

Quantification of the number of viable cells is an indispensable tool in cell biology research. The MTT Cell Proliferation Assay Kit is a sensitive method for quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan, however, dead cells lose this ability. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance (590 nm) is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis.

Product Documents for MTT Cell Proliferation Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MTT Cell Proliferation Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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