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U-2 OS Whole Cell Lysate

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13171

Novus Biologicals, part of Bio-Techne
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NBP2-13171

Key Product Details

Species

Human

Applications

Immunoprecipitation, SDS-PAGE, Western Blot

Product Specifications

Application Notes

Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression, and mass spec. analysis.

Specificity

U-2 OS (human osterogenic sarcoma) whole cell lysate (non-denatured).

Type

Cell

Tissue

Bone/Cartilage

Protein State

Native

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 (without denaturing agents).

Preservative

No Preservative

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: U-2 OS

U2OS is a human bone osteosarcoma from a human 15 year old Caucasian female expressing wild type p53 and lacking p16. U2OS also has a ZFN modification creating a GFP-NUP98 transgene expressed from the endogenous NUP98 gene locus. U2OS cells were established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2OS cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).

Additional U-2 OS Products

Product Documents for U-2 OS Whole Cell Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for U-2 OS Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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