Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel

Name: Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel
Brand: rnd
SKU: LKTM014
Rating: 5 (1 reviews)

Catalog # LKTM014

Citations (6)

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Luminex High Performance Assays

Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.

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Key Product Details

Panel:	Luminex Performance Human Fixed XL Cytokine Magnetic Panel
Assay Type:	Magnetic bead-based multiplex assay for the Luminex^® platform
Format:	1 x 96-well microplate and magnetic antibody-coated beads
Species:	Human
Analytes Detected:	CCL2/JE/MCP-1 CCL3/MIP-1 alpha CCL4/MIP-1 beta CCL5/RANTES CCL11/Eotaxin CCL19/MIP-3 beta CCL20/MIP-3 alpha CD40 Ligand/TNFSF5 CXCL1/GRO alpha/KC/CINC-1 CXCL2/GRO beta/MIP-2/CINC-3 CXCL10/IP-10/CRG-2 EGF FGF basic/FGF2/bFGF Flt-3 Ligand/FLT3L G-CSF GM-CSF Granzyme B IFN-alpha 2/IFNA2 IFN-beta IFN-gamma IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1ra/IL-1F3 IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8/CXCL8 IL-10 IL-12 p70 IL-13 IL-15 IL-17/IL-17A IL-17E/IL-25 IL-33 PD-L1/B7-H1 PDGF-AA PDGF-AB/BB TGF-alpha TNF-alpha TRAIL/TNFSF10 VEGF

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services .

View Full Technical Details for Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel

Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile 44 analytes in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Kit Components

Premixed cocktail of antibody-coated Magnetic beads
Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**
High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays.
	n=40 (20x2 levels)	n=25
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
B7-H1/PD-L1	1.8%	11.3%
CCL11/Eotaxin	3.2%	10.0%
CCL19/MIP-3beta	2.2%	9.7%
CCL2/MCP-1	1.4%	8.9%
CCL20/MIP-3 alpha	2.4%	9.9%
CCL3/MIP-1alpha	1.5%	7.3%
CCL4/MIP-1beta	3.0%	9.6%
CD40 Ligand	3.3%	10.6%
CXCL1/GRO alpha	1.0%	8.0%
CXCL10/IP-10	2.1%	8.8%
CXCL2/GRO beta	1.2%	8.8%
CXCL8/IL-8	1.8%	9.0%
EGF	3.0%	9.6%
FGF-basic	4.2%	12.6%
Flt-3 ligand	2.5%	10.1%
G-CSF	1.8%	10.2%
GM-CSF	3.1%	10.3%
Granzyme B	1.8%	11.0%
IFN-alpha	2.9%	9.9%
IFN-beta	3.0%	11.0%
IFN-gamma	2.8%	10.4%
IL-10	3.3%	9.0%
IL-12 p70	2.3%	10.5%
IL-13	1.6%	9.7%
IL-15	2.4%	9.9%
IL-17A	2.5%	11.2%
IL-17E/IL-25	1.7%	9.2%
IL-1alpha	3.0%	10.4%
IL-1beta	2.3%	10.0%
IL-1ra	2.1%	10.3%
IL-2	2.2%	9.2%
IL-3	2.5%	10.1%
IL-33	2.4%	12.3%
IL-4	3.2%	9.7%
IL-5	2.2%	12.0%
IL-6	2.6%	9.8%
IL-7	2.5%	9.8%
PDGF-AA	2.8%	15.5%
PDGF-AB/BB	2.4%	12.4%
RANTES	4.7%	17.4%
TGF-alpha	3.0%	9.8%
TNF-alpha	2.2%	8.7%
TRAIL	2.0%	10.3%
VEGF	2.6%	10.3%

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an astrisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
	Linearity		Spiked Recovery
Analyte	N	Average %	N	Average %
*Natural linearity
B7-H1/PD-L1	4	106%	4	109%
CCL11/Eotaxin	4	92%	4	107%
CCL19/MIP-3beta	4	123%	4	95%
CCL2/MCP-1	4*	82%		High endogenous
CCL20/MIP-3 alpha	4	107%	4	99%
CCL3/MIP-1alpha	4	77%	4	139%
CCL4/MIP-1beta	4	103%	4	111%
CD40 Ligand	4	113%	4	100%
CXCL1/GRO alpha	4	80%		High endogenous
CXCL10/IP-10	3	110%		High endogenous
CXCL2/GRO beta	4*	114%	3	106%
CXCL8/IL-8	4	118%	4	96%
EGF	4*	110%	4	106%
FGF-basic	4	161%	3	80%
Flt-3 ligand	4	102%	4	109%
G-CSF	4	209%	4	43%
GM-CSF	4	96%	4	110%
Granzyme B	4	99%	4	118%
IFN-alpha	3	98%	4	101%
IFN-beta	4	148%	4	69%
IFN-gamma	4	97%	4	112%
IL-10	4	111%	4	104%
IL-12 p70	4	106%	4	108%
IL-13	4	112%	4	103%
IL-15	3	88%	3	89%
IL-17A	4	110%	4	106%
IL-17E/IL-25	4	122%	4	87%
IL-1alpha	4	143%	4	80%
IL-1beta	4	107%	4	99%
IL-1ra	4*	100%	4	145%
IL-2	4	105%	4	102%
IL-3	4	123%	4	88%
IL-33	4	93%	4	110%
IL-4	3	127%	3	77%
IL-5	3	113%	3	81%
IL-6	3	124%	3	90%
IL-7	4	113%	4	96%
PDGF-AA	4*	90%		High endogenous
PDGF-AB/BB	4*	96%		High endogenous
RANTES	4*	96%		High endogenous
TGF-alpha	4	117%	4	96%
TNF-alpha	4	117%	4	69%
TRAIL	3	123%	4	88%
VEGF	4	103%	4	109%

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel Scientific Data Examples

	Human XL Cytokine Luminex Panel Standard Curves
	Human XL Cytokine Performance Luminex Panel Serum Sample Values Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Citations for Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel (6)

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Citations are publications that use Bio-Techne products. Selected citations for Human XL Cytokine Magnetic Luminex Performance Assay 44-plex Fixed Panel include:

Species: Human
Sample Types: Plasma

A Venkataram et al. (2021), Plasma biomarker profiling of PIMS-TS, COVID-19 and SARS-CoV2 seropositive children - a cross-sectional observational study from southern India EBioMedicine, 66(0):103317.
PMID: 33813138
Species: Human
Sample Types: Cell Culture Supernates

NM Schmidt et al. (2021), Targeting human Acyl-CoA:cholesterol acyltransferase as a dual viral and Tï¿½cell metabolic checkpoint Nature Communications, 12(1):2814.
PMID: 33990561
Species: Human
Sample Types: Plasma

L Giloteaux et al. (2020), Cytokine profiling of extracellular vesicles isolated from plasma in myalgic encephalomyelitis/chronic fatigue syndrome: a pilot study J Transl Med, 18(1):387.
PMID: 33046133
Species: Human
Sample Types: Plasma

M Blot et al. (2020), The dysregulated innate immune response in severe COVID-19 pneumonia that could drive poorer outcome Journal of Translational Medicine, 18(1):457.
PMID: 33272291
Species: Human
Sample Types: BALF

M Blot et al. (2020), CXCL10 could drive longer duration of mechanical ventilation during COVID-19 ARDS Crit Care, 24(1):632.
PMID: 33138839
Species: Human
Sample Types: Plasma

J Balnis et al. (2020), Higher plasma levels of Chemokine CCL19 are associated with poor SARS-CoV-2 acute respiratory distress syndrome (ARDS) outcomes medRxiv, 0(0).
PMID: 32511515

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Human XL Cytokine Magnetic Luminex Performance Assay 45-plex Fixed Panel

Name: Anonymous

Sample Tested: Cerebrospinal Fluid

Verified Customer | Posted 11/24/2020

The human XL Cytokine fixed panel performed as expected with low % CV's for all analytes

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