Are QC controls available for both the Luminex Discovery and High Performance Assays?

Controls are not offered for Luminex Discovery Assays, but may be formulated by the end user. A general guide may be available upon request. Controls are available for select Luminex High Performance Assays and can be found under the supplemental products tab on the product webpage. Some High Performance Luminex kits include controls and the controls will be listed in the materials provided section on the product datasheet. When included in the kit, controls are not sold separately.

At what frequency should Calibration and Verification tests be performed?

It is recommended that a Calibration test be performed within one week prior to running an assay. A Verification test should be performed prior to data acquisition on the day of running an assay.

Does the Human Luminex Discovery Assay detect soluble form or membrane-bound analyte?

The sample types typically validated by R&D Systems for Luminex assays contain soluble form of proteins, and therefore it is expected that the soluble form of proteins are detected. It is possible there are assays that also detect the membrane form of respective target proteins, however, R&D Systems does not generally evaluate our assays against samples known to contain membrane-bound proteins.

Is the reconstituted standard a concentrate?

For Discovery Assays, each standard cocktail is a concentrate upon reconstitution with the volume specified on the Certificate of Analysis (CoA). After creating Standard 1 following the table in the product insert, the Standard 1 value is the concentration listed on the CoA.

What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?

R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts.

What speed do you recommend centrifuging cell culture supernates samples?

For cell culture supernates, remove particulates by centrifuging for 5 minutes at 500 x g.

Why does the Luminex Assay Customization Tool provide the option to split based on dilution?

The feature to split based on dilution enables investigators to configure premix Luminex panels based on sample type (cell culture supernates, serum, and plasma). This keeps analytes requiring the same dilution factor within a single panel, while separating analytes requiring different dilutions. The recommended dilution factor for each analyte may be different for each sample type, and the suggested sample dilutions are built into the Luminex Customization Tool. Note that the suggested dilution factors are based on samples from healthy volunteers.

Human Luminex® Discovery Assay Best Seller

Name: Human Luminex® Discovery Assay
Brand: R&D Systems
SKU: LXSAHM
Rating: 4 (41 reviews)

R&D Systems, part of Bio-Techne | Catalog # LXSAHM

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Citations (1062)

Product Datasheet / COA / SDS

Luminex Discovery Assays

Human Luminex® Discovery Assay is designed for detecting 360 Human analytes in serum, plasma, or tissue culture supernatants simultaneously. The Luminex Discovery Assays provide extensive flexibility for multiplexing. These assays are fully customizable, offering a selection of analytes to suit individual research needs.

Explore customization options and build your panel with the Luminex Assay Customization Tool.

Build Now

Build Your Assay
Product Features
Performance Validation
Kit Contents
Which Luminex Assay Is Right For Me
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Magnetic bead-based multiplex assay for the Luminex® platform

Format

1 x 96-well microplate and magnetic antibody-coated beads

Species

Human

Analytes Detected

Select from over 360 analytes to build your custom panel using our assay customization tool below.

Build Your Assay

Make Selections Below to Configure Your Custom Luminex® Assay

Configured Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If overlapping bead regions occur in analyte selections, send your Luminex configuration code to our team for resolution.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on sample dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Product Features

Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Simultaneously profile your selection of analytes in one sample.
Choose from a wide menu of analytes, including many unique analytes
Requires a small sample volume (<50 µL)
Run your assay in just 3-4 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate
Quick turn-around time: Kits are typically shipped within 5-10 days of ordering*

*Please allow additional time for international orders.

Performance Validation

R&D Systems Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Kit Contents

Premixed cocktail of antibody-coated Magnetic beads
Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Which Luminex Assay is Right for Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays .

High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.

Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.

Additional Information About Luminex Assays

All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.

Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.

Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex^® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Human Luminex® Discovery Assay

Product Datasheets

Download Product Datasheet

Download Product Datasheet Human Discovery Datasheet Rev 22 for Standards A - Z and 1 - 7

COA

SDS

Download Human Discovery SDS for Standards 8 - 23

Download Human Discovery SDS for Standards A - Z and 1 - 7

Download SDS

Product Specific Notices for Human Luminex® Discovery Assay

Luminex® is a registered trademark of Luminex Corporation.

For research use only

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