Rat IgG2a Lambda Isotype Control (RG2aL)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31382
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Lambda Clone # RG2aL
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
Isotyping assay resulted non-reactive with antisera to Rat IgG1a, IgG1b, IgG3, IgM, and IgA
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Lambda
Description
This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG1 and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1a, IgG1b, IgG3, IgM, and IgA. Light and heavy chain composition has been confirmed by SDS-PAGE.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Applications for Rat IgG2a Lambda Isotype Control (RG2aL)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:1000 - 1:5000
Fluorophore-linked immunosorbent assay
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgG2a Lambda
Additional IgG2a Lambda Products
Product Documents for Rat IgG2a Lambda Isotype Control (RG2aL)
Product Specific Notices for Rat IgG2a Lambda Isotype Control (RG2aL)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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