Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49784
Discontinued Product
NBP2-49784 has been discontinued.
View all Exosome Capture (Biofluids, Cell Media) and RNA products.
Key Product Details
Species
Human
Kit Type
Extraction Kit
Product Summary for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
Fast and user friendly protocol
Small starting amount of sample
Better RNA yield than similar products
Exosome Standards included as control
RNA extraction from overall exosome population captured with immunobeads
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab287871)
Small starting amount of sample
Better RNA yield than similar products
Exosome Standards included as control
RNA extraction from overall exosome population captured with immunobeads
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab287871)
Product Specifications
Application Notes
Direct capture and exosome RNA extraction from human biofluids and cell culture media without initial exosome purification step
Starting from 0.1 ml of biological sample (plasma) per reaction. Whole plasma and serum can be directly used for capture. Concentrated urine and cell culture supernatant samples are recommended prior capture according to our suggested protocol, Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray).
Starting from 0.1 ml of biological sample (plasma) per reaction. Whole plasma and serum can be directly used for capture. Concentrated urine and cell culture supernatant samples are recommended prior capture according to our suggested protocol, Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray).
Reactivity Notes
Exosomal RNA isolation from human biological fluids including plasma, serum, urine and cell culture media.
Scientific Data Images for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
Overall Exosome Capture and RNA Extraction (Biofluids, Cell Media) Kit [NBP2-49784] - Expected Nanodrop profile for RNA extracted from immunocaptured exosomes (100 ul of human plasma). Yield = 8.4 ng/ul; A260/280 =1.6; A260/230= 1.85
Overall Exosome Capture and RNA Extraction (Biofluids, Cell Media) Kit [NBP2-49784] - Comparasion of total RNA yield (Nanodrop quantification) for exosomal RNA extraction kits from Novus Biologicals' and a competitor
Kit Contents for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
- 1.5 ml RNase free elution tubes
- 5X Bead Washing buffer
- Columns
- Elution buffer
- Lyophilized Exosome Standard
- Lysis buffer
- Pre-couple latex immunobeads
- RNA Washing buffer (to add Ethanol 96%)
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C. Do not freeze.
Background: Exosome Capture (Biofluids, Cell Media) and RNA
Alternate Names
exosome capture and quantification
Product Documents for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
Product Specific Notices for Exosome Capture (Biofluids, Cell Media) and RNA Extraction Kit
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...
Loading...