Animal Blood B Booster Kit

Novus Biologicals, part of Bio-Techne | Catalog # DDXK-AnBBB

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
DDXK-AnBBB
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species

Mouse, Rat, Canine, Equine, Rabbit, Sheep

Applications

Functional Assay

Kit Type

Kit

Product Summary for Animal Blood B Booster Kit

This kit is an extension of the Human Blood B Booster Kit (DDXK-HuBBB) to animal species. Used in combination with the murine myeloma SP2/0 cell line, the Animal Blood B Booster kit (DDXK-AnBBB) is intended for immortalization of B lymphocytes isolated from a wide range of animal species (validated in horse, rabbit, sheep, rat, dog).

Product Specifications

Application Notes

This kit is an extension of the Human Blood B Booster Kit (DDXK-HuBBB) to animal species. Used in combination with the murine myeloma SP2/0 cell line, the Animal Blood B Booster kit (DDXK-AnBBB) is intended for immortalization of B lymphocytes isolated from a wide range of animal species (validated in horse, rabbit, sheep, rat, dog).

Scientific Data Images for Animal Blood B Booster Kit

B Cell Stimulation: Animal Blood B Booster Kit [DDXK-AnBBB] - Microscopic observation of canine lymphocyte activation after the first stimulation.
B Cell Stimulation: Animal Blood B Booster Kit [DDXK-AnBBB] - Microscopic observation of canine lymphocyte activation after 2nd stimulation.

Kit Contents for Animal Blood B Booster Kit

  • Booster reagent - 67 ml
  • Vial #1 - support 1 (Lyophilized)
  • Vial #2 - mAb1 (200 ul)
  • Vial #3 - mAb2 (200 ul)
  • Vial #4 - support2 (Lyophilized)
  • Vial #5 - mAb3 (200 ul)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: Animal Blood B Booster

Dendritics routinely uses the hybridoma technology described by Kohler and Milstein, where the freshly isolated splenocytes of immunized mice, are fused with the Ig non secreting murine myeloma SP2/0 cell line to immortalize Ig secreting B lymphocytes. The resulting repertoire is generally limited either by the low immunogenicity of the antigen, or by the natural immunity of the mouse. Because the Blood B Booster system (DDXK-HuBBB) was demonstrated as being powerful for human B cells, we attempted to improve the efficiency of the hybridoma technology by introducing an activation step before the fusion in an attempt to give access to a broader repertoire. Briefly, after the immunization program, freshly isolated splenocytes were separately stimulated according to two stimulation systems based on B cell activation through CD40 triggering. We have assumed that the inter-species homology of the CD40 antigen will allow non human B cell activation by a monoclonal antibody raised against human CD40. After the demonstration that the introduction of the stimulation step before fusion significantly increased the clonal diversity of the immortalized murine B cell populations, this process was successfully extended to other animal species starting from peripheral blood samples (Razanajaona-Doll D et al, AAI 98th annual meeting, Immunology 2011TM, San Francisco, CA, USA). The kit AnBBB(R) was packaged so as to make it very easy to handle for the scientists ; the kit AnBBB(R) contains all the reagents needed to obtain around 3.103 independent B cell clones from 6.107 splenocytes or PBMC using six 96 wells culture plates for the stimulations and ten 96 wells plates for the fusion step. The resulting cells are available for a wide range of studies including genomic, transcriptomic and proteomic fields.

Alternate Names

Animal B cell immortalization, Animal B cell immortalization Animal Blood B Booster, Animal Blood B Booster kit, Blood B Booster, Blood B Booster Kit

Additional Animal Blood B Booster Products

Product Documents for Animal Blood B Booster Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Animal Blood B Booster Kit

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.