AbSelect (TM) Mouse TCS Antibody Purification Kit
Novus Biologicals, part of Bio-Techne | Catalog # 832-0500
Discontinued Product
832-0500 has been discontinued.
View all AbSelect (TM) Mouse TCS products.
Key Product Details
Kit Type
Antibody Purification Kit
Product Summary for AbSelect (TM) Mouse TCS Antibody Purification Kit
The AbSelect Mouse TCS Purification System enables the selective purification of mouse IgG from hybridoma supernatants without the co-purification of bovine IgG. This is because AbSelect Mouse resin has a high affinity and specificity for mouse IgG molecules whereas the binding strength of bovine IgG to AbSelect Mouse resin is negligible. In comparison, Protein G does not have the capability to selectively purify mouse IgG from hybridoma supernatants.
The method of AbSelect Mouse TCS Purification System involves capture of the antibody on the AbSelect Mouse resin and the removal of unwanted substances using a simple wash procedure. The purified antibody is then eluted and neutralized. AbSelect Mouse TCS Purification System is not suitable for use with antibodies from other species.
The components of all AbSelect kits are fully compatible with the Lightning-Link and Lightning-Link Rapid antibody labeling systems (available separately), which allow the purified antibody to be rapidly conjugated to a wide variety of labels.
The method of AbSelect Mouse TCS Purification System involves capture of the antibody on the AbSelect Mouse resin and the removal of unwanted substances using a simple wash procedure. The purified antibody is then eluted and neutralized. AbSelect Mouse TCS Purification System is not suitable for use with antibodies from other species.
The components of all AbSelect kits are fully compatible with the Lightning-Link and Lightning-Link Rapid antibody labeling systems (available separately), which allow the purified antibody to be rapidly conjugated to a wide variety of labels.
Product Specifications
Application Notes
The mouse antibody to be purified should be in 10 to 25ml of tissue culture supernatant. Up to 1.5mg of antibody can be purified in each run.
Scientific Data Images for AbSelect (TM) Mouse TCS Antibody Purification Kit
AbSelect Mouse TCS System Antibody Purification Kit [832-0500]
Kit Contents for AbSelect (TM) Mouse TCS Antibody Purification Kit
- 1 or 3 spin cartridge / collecting tube assemblies
- 1 or 3 vials of AbSelect Mouse resin
- 1 vial of 10x Binding buffer
- 1 vial of Elution buffer
- 1 vial of Neutralization buffer
- 1 vial of Washing buffer
- 4 or 12 additional collecting tubes
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C.
Product Documents for AbSelect (TM) Mouse TCS Antibody Purification Kit
Product Specific Notices for AbSelect (TM) Mouse TCS Antibody Purification Kit
AbSelect® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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