MitoMark Green I
Tocris Bioscience, part of Bio-Techne | Catalog # 6444
Green fluorescent mitochondrial stain; cell permeable
Discontinued Product
6444 has been discontinued.
View all Other Mitochondria Dye Compounds products.
Key Product Details
Description
Green fluorescent mitochondrial stain; cell permeable
Product Description
MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.It is recommended to prepare stock solutions in DMSO.
Scientific Data Images for MitoMark Green I
Applications in HeLa cells for MitoMark Green I.
Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.Optical Data
| Emission Color | Green |
| λabs | 490 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 119000 M-1cm-1 |
Spectra Viewer
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Spectra ViewerProduct Specifications
Molecular Weight
671.87
Formula
C34H28Cl5N3O
Storage
Store at -20°C
Purity
≥85% (HPLC)
Chemical Name
2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride
CAS Number
201860-17-5
PubChem ID
77913543
InChI Key
RUVJFMSQTCEAAB-UHFFFAOYSA-M
SMILES
ClC1=CC2=C([N+](CC3=CC=C(CCl)C=C3)=C(/C=C/C=C4N(C)C5=CC=CC=C5O\4)N2CC6=CC=C(CCl)C=C6)C=C1Cl.[Cl-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Garner Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol.Reprod. 1997 PMID: 9408246
Product Specific Notices for MitoMark Green I
For research use only
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