Cyanine 3, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 5435
Orange fluorescent dye for labeling of amines; supplied as NHS ester
Discontinued Product
5435 has been discontinued.
View all Fluorescent Dyes products.
Key Product Details
Description
Orange fluorescent dye for labeling of amines; supplied as NHS ester
Product Description
Cyanine 3, SE is an orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.Optical Data
| Emission Color | Orange |
| Brightness | |
| λabs | 550 nm |
| λem | 570 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.09 |
| Application | Flow Cytometry |
| Alternative For | Alexa Fluor 546; DyLight 549 |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
727.84
Formula
C35H41N3O10S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-[3-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
CAS Number
146368-16-3
PubChem ID
91811030
InChI Key
SOVMUUCAZOFIQP-UHFFFAOYSA-N
SMILES
CC[N+]1=C(C(C)(C)C2=C1C=CC(S(=O)([O-])=O)=C2)/C=C/C=C3N(CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC(S(=O)(O)=O)=C5)=C5C/3(C)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 72.78 | 100 | |
| DMSO | 72.78 | 100 |
Preparing Stock Solutions
for Cyanine 3, SE
The following data is based on the product molecular weight 727.84
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.37 mL | 6.87 mL | 13.74 mL |
| 5 mM | 0.27 mL | 1.37 mL | 2.75 mL |
| 10 mM | 0.14 mL | 0.69 mL | 1.37 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.27 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Shin Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 2014 PMID: 24753407
Product Specific Notices for Cyanine 3, SE
For research use only
Loading...
Loading...
Loading...