7-Aminoactinomycin D

Name: 7-Aminoactinomycin D
Brand: Tocris Bioscience
SKU: 7121
Price: 168 USD

Catalog # 7121 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Fluorescent DNA stain

Key Product Details

Description:	Fluorescent DNA stain
Chemical Name:	2,7-Diamino-N¹,N⁹-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Purity:	≥97% (HPLC)
Molecular Weight:	1270.45

View Full Technical Details for 7-Aminoactinomycin D

Biological Activity for 7-Aminoactinomycin D

7-Aminoactinomycin D is a fluorescent DNA stain. G-C base-specific DNA intercalator that forms stable complexes with DNA. Suitable for use in flow cytometry. Detects apoptotic cells. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Excitation/emission maxima (λ) = 488/655 nm.

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Technical Data for 7-Aminoactinomycin D

M. Wt	1270.45
Formula	C₆₂H₈₇N₁₃O₁₆
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	7240-37-1
PubChem ID	14924508
InChi Key	YXHLJMWYDTXDHS-UHFFFAOYSA-N
Smiles	NC1=C(C(NC(C(C)OC(C(C(C)C)N(C(CN(C)C2=O)=O)C)=O)C(NC(C(C)C)C(N3C2CCC3)=O)=O)=O)C4=NC5=C(C(NC(C(NC(C(C)C)C6=O)=O)C(C)OC(C(C(C)C)N(C(CN(C)C(C7N6CCC7)=O)=O)C)=O)=O)C=C(N)C(C)=C5OC4=C(C)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 7-Aminoactinomycin D

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	2

Preparing Stock Solutions for 7-Aminoactinomycin D

The following data is based on the product molecular weight 1270.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.02 mM	39.36 mL	196.78 mL	393.56 mL
0.1 mM	7.87 mL	39.36 mL	78.71 mL
0.2 mM	3.94 mL	19.68 mL	39.36 mL
1 mM	0.79 mL	3.94 mL	7.87 mL

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References for 7-Aminoactinomycin D

References are publications that support the biological activity of the product.

Philpott The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques. Blood 1996 PMID: 8630384
Schmid Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 1992 PMID: 1547670
Zelenin 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry 1984 PMID: 6468175

Citations for 7-Aminoactinomycin D

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