5-TAMRA NHS
Tocris Bioscience, part of Bio-Techne | Catalog # 7665
Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM
Discontinued Product
7665 has been discontinued.
View all Fluorescent Probes products.
Key Product Details
Description
Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM
Product Description
Key information: 5-TAMRA NHS is an orange fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Can be used for oligonucleotide labeling.Application: Suitable for fluorescence microscopy, confocal microscopy, FRET acceptor for FAM.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 546 nm and 580 nm, respectively; quantum yield = 0.1; extinction coefficient = 95,000 M-1cm-1; A280 = 0.178.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to prepare stock solutions in DMSO.
Optical Data
| Emission Color | Orange |
| Brightness | |
| λabs | 546 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95,000 M-1cm-1 |
| Quantum Yield (φ) | 0.1 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| pH Sensitivity | No |
| Correction Factor 280 | 0.178 |
| Application | Fluorescence Microscopy, Confocal Microscopy |
| Alternative For | Alexa Fluor® 546 |
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Product Specifications
Molecular Weight
527.52
Formula
C29H25N3O7
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
CAS Number
150810-68-7
PubChem ID
2762600
InChI Key
VWFRSNKRTNUMET-UHFFFAOYSA-N
SMILES
CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Hirata Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 2014 PMID: 25036716
- Kvach Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 2009 PMID: 19606815
Product Specific Notices for 5-TAMRA NHS
For research use only
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