3-Cyano-7-ethoxycoumarin
Tocris Bioscience, part of Bio-Techne | Catalog # 4815
Fluorescent cytochrome P450 substrate
Discontinued Product
4815 has been discontinued.
View all Fluorescent Enzyme Probes and Enzyme Substrates products.
Key Product Details
Description
Fluorescent cytochrome P450 substrate
Product Description
3-Cyano-7-ethoxycoumarin is a fluorescent P450 substrate (excitation/emission wavelengths = 408/455 nm); metabolized to cyano-hydroxycoumarin.Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra ViewerProduct Specifications
Molecular Weight
215.20
Formula
C12H9NO3
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
7-Ethoxy-2-oxo-2H-1-benzopyran-3-carbonitrile
CAS Number
117620-77-6
PubChem ID
164045
InChI Key
YAFGHMIAFYQSCF-UHFFFAOYSA-N
SMILES
O=C2C(C#N)=CC1=CC=C(OCC)C=C1O2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 50 |
Preparing Stock Solutions
for 3-Cyano-7-ethoxycoumarin
The following data is based on the product molecular weight 215.20
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 9.29 mL | 46.47 mL | 92.94 mL |
| 2.5 mM | 1.86 mL | 9.29 mL | 18.59 mL |
| 5 mM | 0.93 mL | 4.65 mL | 9.29 mL |
| 25 mM | 0.19 mL | 0.93 mL | 1.86 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Donato Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Drug Metab.Dispos. 2004 PMID: 15205384
- White A continuous fluorometric assay for cytochrome P-450-dependent mixed function oxidases using 3-cyano-7-ethoxycoumarin. Anal.Biochem. 1988 PMID: 3189781
Product Specific Notices for 3-Cyano-7-ethoxycoumarin
For research use only
Loading...
Loading...