Human Vitamin B12 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60196

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

1.498 pg/mL

Assay Range

1.56 pg/mL - 100 pg/mL

View all Vitamin B12 ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human VB12. No significant cross-reactivity or interference between human VB12 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human Vitamin B12 ELISA Kit (Colorimetric)

ELISA: Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196]

ELISA: Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196]

ELISA: Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Vitamin B12 ELISA Kit (Colorimetric)

Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196] -

Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196] - Standard Curve Reference

Kit Contents for Human Vitamin B12 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • Biotin-conjugate (10 x concentrate)
  • Biotin-conjugate Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitamin B12

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional Vitamin B12 Products

Product Documents for Human Vitamin B12 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Vitamin B12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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