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Rat Vitamin A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60194

Novus Biologicals, part of Bio-Techne
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NBP2-60194-1Kit

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

233.598 pg/mL

Assay Range

312.5 pg/mL - 5000 pg/mL

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This assay has high sensitivity and excellent specificity for detection of rat VA. No significant cross-reactivity or interference between rat VA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Rat Vitamin A ELISA Kit (Colorimetric)

Rat Vitamin A ELISA Kit (Colorimetric)

Rat Vitamin A ELISA Kit (Colorimetric) [NBP2-60194] -

Rat Vitamin A ELISA Kit (Colorimetric) [NBP2-60194] - Standard Curve Reference

Kit Contents for Rat Vitamin A ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (10 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitamin A

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.

Additional Vitamin A Products

Product Documents for Rat Vitamin A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Vitamin A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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