Fish Triiodothyronine ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60182

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.5 ng/mL

Assay Range

0.5 ng/mL - 8 ng/mL

View all Triiodothyronine ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Fish

Specificity

This assay has high sensitivity and excellent specificity for detection of fish T3. No significant cross-reactivity or interference between fish T3 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Fish Triiodothyronine ELISA Kit (Colorimetric)

ELISA: Fish Triiodothyronine ELISA Kit (Colorimetric) [NBP2-60182]

ELISA: Fish Triiodothyronine ELISA Kit (Colorimetric) [NBP2-60182]

ELISA: Fish Triiodothyronine ELISA Kit (Colorimetric) [NBP2-60182] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Fish Triiodothyronine ELISA Kit (Colorimetric)

Fish Triiodothyronine ELISA Kit (Colorimetric) [NBP2-60182] -

Fish Triiodothyronine ELISA Kit (Colorimetric) [NBP2-60182] - Standard Curve Reference

Kit Contents for Fish Triiodothyronine ELISA Kit (Colorimetric)

  • Assay plate
  • Conjugate
  • HRP-avidin
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Triiodothyronine

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

liothyronine; T3

Additional Triiodothyronine Products

Product Documents for Fish Triiodothyronine ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fish Triiodothyronine ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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