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Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # KA0459

Catalog #
Size / Price

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Cell Lysate, CSF, Milk, Plasma, Saliva, Serum, Urine (50 uL)


0.31 ng/mL

Assay Range

0.391 to 25 ng/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)




SERPINA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SERPINA1.


Intra-Assay Precision (Precision within an assay) CV% < 4.10%

Inter-Assay Precision (Precision between assays) CV% < 7.10%

Recovery for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)



Scientific Data Images for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

ELISA: Serpin A1/alpha 1-Antitrypsin ELISA Kit [Biotin] [KA0459] - Standard curve

Kit Contents for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

  • Biotinylated Human alpha-1-Antitrypsin Antibody (70x): A 70-fold biotinylated polyclonal antibody against human A1AT.
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • Human alpha-1-Antitrypsin Microplate: A polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human A1AT.
  • Human alpha-1-Antitrypsin Standard: Human A1AT in a buffered protein base, lyophilized.
  • MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage


The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Serpin A1/alpha 1-Antitrypsin

The SERPINA1 (Human) ELISA Kit is designed for detection of A1AT in human urine, milk, saliva, CSF and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures A1AT in less than 4 hours. A polyclonal antibody specific for A1AT has been pre-coated onto a 96-well microplate with removable strips. Human A1AT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for A1AT, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Gene Symbol


Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.