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Chicken Progesterone ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23550

Novus Biologicals, part of Bio-Techne
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NBP3-23550

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 uL)

Sensitivity

0.06 ng/mL

Assay Range

0.16-10 ng/mL

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Chicken

Specificity

This kit recognizes Chicken Progesterone in samples. No significant cross-reactivity or interference between Chicken Progesterone and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.76

Inter-Assay Precision (Precision between assays) %CV < 5.57

Recovery for Chicken Progesterone ELISA Kit (Colorimetric)

Recovery

89-104%

Linearity

Scientific Data Images for Chicken Progesterone ELISA Kit (Colorimetric)

Chicken Progesterone ELISA Kit (Colorimetric)

ELISA: Chicken Progesterone ELISA Kit (Colorimetric) [NBP3-23550] -

ELISA: Chicken Progesterone ELISA Kit (Colorimetric) [NBP3-23550] - Standard Curve Reference

Kit Contents for Chicken Progesterone ELISA Kit (Colorimetric)

  • Concentrated HRP Linked Detection Ab (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Linked Ab Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Progesterone

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Chicken Progesterone ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Chicken Progesterone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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