Bovine Progesterone ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60122

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.2 ng/mL

Assay Range

1 ng/mL - 70 ng/mL

View all Progesterone ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Bovine

Specificity

This assay has high sensitivity and excellent specificity for detection of bovine PROG. No significant cross-reactivity or interference between bovine PROG and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Bovine Progesterone ELISA Kit (Colorimetric)

ELISA: Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122]

ELISA: Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122]

ELISA: Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Bovine Progesterone ELISA Kit (Colorimetric)

Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] -

Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] - Standard Curve Reference

Kit Contents for Bovine Progesterone ELISA Kit (Colorimetric)

  • Adhesive Cover Strip (for 96 wells)
  • Antibody
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20X concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Progesterone

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Bovine Progesterone ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bovine Progesterone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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