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Mouse/Rat FABP1/L-FABP DuoSet ELISA

Catalog # DY1565-05 | R&D Systems, Inc. a Bio-Techne Brand
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DY1565-05

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

125-8000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse, Rat

Mouse/Rat FABP1/L-FABP DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Mouse/Rat FABP1/L-FABP DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse and rat Fatty Acid-Binding Protein 1 (FABP-1). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Mouse/Rat FABP1/L-FABP DuoSet ELISA

Mouse/Rat FABP-1/L-FABP ELISA Standard Curve

Mouse/Rat FABP-1/L-FABP ELISA Standard Curve

Kit Contents for Mouse/Rat FABP1/L-FABP DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP1/L-FABP

Fatty Acid Binding Protein 1, also known as liver FABP, is a cytoplasmic transporter for long chain fatty acids, oxidized/peroxidized fatty acids, cholesterol, heme, Vitamin K, steroids, acyl CoA, leukotrienes, and prostaglandins. It is expressed by hepatocytes, simple columnar epithelium of the small intestine, and renal proximal tubule epithelium. FABP1 trafficks between the cytosol, peroxisomes, and nucleus. It delivers fatty acid cargo to the transcription factor PPAR alpha, leading to increased production of enzymes involved in the beta-oxidation of fatty acids. FABP1 also plays a role in the formation of prechylomicron vesicles by the endoplasmic reticulum.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

Additional FABP1/L-FABP Products

Product Documents for Mouse/Rat FABP1/L-FABP DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse/Rat FABP1/L-FABP DuoSet ELISA

For research use only

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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