Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # MLCN20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Urine (10 µL)
Sensitivity
8.8 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Product Summary for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Lipocalin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Platelet-poor Plasma/Urine - Samples were evaluated for the presence of mouse
Lipocalin-2 in this assay.
Cell Culture Supernates:
Two mouse brains and kidneys were homogenized and seeded in RPMI 1640 supplemented with 10% fetal bovine serum, 5 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL of penicillin, and 100 μg/mL of streptomycin sulfate. The resulting supernates were either untreated or stimulated with 1 μg/mL of LPS for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of mouse Lipocalin-2.
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=20) | 141 | 58.3-290 | 60.2 |
| Platelet-poor heparin plasma (n=20) | 76.0 | 40.7-143 | 30.1 |
| Platelet-poor EDTA plasma (n=20) | 54.1 | 32.5-74.7 | 12.3 |
| Urine (n=20) | 48.6 | 12.9-168 | 34.1 |
Cell Culture Supernates:
J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106
cells/mL) were cultured in
RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL of penicillin,
and 100 μg/mL of streptomycin sulfate. Cells were either untreated or stimulated with
100 ng/mL of recombinant mouse IFN-gamma and 1 μg/mL of lipopolysaccharide (LPS) for 2 days.
Aliquots of the cell culture supernates were removed and assayed for levels of mouse
Lipocalin-2.
| Condition | Observed Values (ng/mL) |
| Unstimulated | 0.817 |
| Stimulated | 161 |
WEHI-3 myelomonocytic leukemia cells (2 x 105
cells/mL) were cultured in RPMI supplemented
with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL of penicillin, and 100 μg/mL of
streptomycin sulfate. Cells were either untreated or stimulated with 1 μg/mL of LPS for 4 days.
Aliquots of the cell culture supernates were removed and assayed for levels of mouse
Lipocalin-2.
| Condition | Observed Values (ng/mL) |
| Unstimulated | 83.7 |
| Stimulated | 178 |
Two mouse brains and kidneys were homogenized and seeded in RPMI 1640 supplemented with 10% fetal bovine serum, 5 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL of penicillin, and 100 μg/mL of streptomycin sulfate. The resulting supernates were either untreated or stimulated with 1 μg/mL of LPS for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of mouse Lipocalin-2.
| Condition | Observed Values (ng/mL) |
| Brain unstimulated | 0.334 |
| Brain stimulated | 178 |
| Condition | Observed Values (ng/mL) |
| Kidney Unstimulated | 1.28 |
| Kidney Stimulated | 4.69 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 196 | 476 | 1534 | 211 | 477 | 1564 |
| Standard Deviation | 13.2 | 19.6 | 65.9 | 15.2 | 28.6 | 98.5 |
| CV% | 6.7 | 4.1 | 4.3 | 7.2 | 6.0 | 6.3 |
Recovery for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
The recovery of mouse Lipocalin-2 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 93 | 82-101 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Lipocalin-2 were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
Mouse Lipocalin-2/NGAL ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Lipocalin-2/NGAL
Lipocalin-2/NGAL (Neutrophil Gelatinase-associated Lipocalin) is a secreted carrier protein that acts in the innate immune response, differentiation, tumorigenesis, and cell survival. It is induced in macrophages, glial cells, and epithelial cells during inflammation, anemia, and hypoxia. Lipocalin-2 binds catecholate siderophores which are secreted by bacteria and are required for bacterial iron uptake. Lipocalin-2 functions as a bacteriostatic agent in the innate immune response by limiting the bacterial iron supply. Iron uptake by Lipocalin-2 into mammalian cells is important for the regulation of iron-sensitive gene transcription. In the kidney, Lipocalin-2-mediated iron trafficking is required for protection from renal injury.
Long Name
Neutrophil Gelatinase-associated Lipocalin
Alternate Names
24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin
Gene Symbol
LCN2
Additional Lipocalin-2/NGAL Products
Product Documents for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
Product Specific Notices for Mouse Lipocalin-2/NGAL Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.