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Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39771

Novus Biologicals, part of Bio-Techne
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NBP3-39771

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

5.9 pg/mL (example only; lot dependent)

Assay Range

15.625 - 1000 pg/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images

Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric)

ELISA: Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric) [NBP3-39771]

ELISA: Human Macrophage Inflammatory Protein 1 alpha ELISA Kit (Colorimetric) [NBP3-39771] - Standard Curve Reference

Kit Contents

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Macrophage Inflammatory Protein 1 alpha

Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.

Alternate Names

C-C motif chemokine 3, chemokine (C-C motif) ligand 3, G0S19-1Tonsillar lymphocyte LD78 alpha protein, LD78ALPHA, Macrophage inflammatory protein 1-alpha, MIP1AG0/G1 switch regulatory protein 19-1, MIP-1-alphaSIS-beta, SCYA3PAT 464.1, small inducible cytokine A3 (homologous to mouse Mip-1a), Small-inducible cytokine A3

Gene Symbol

CCL3

Additional Macrophage Inflammatory Protein 1 alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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