Human Total SMAC/Diablo DuoSet IC ELISA

Name: Human Total SMAC/Diablo DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC789-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC789-2

Citations (1)

Product Datasheet / COA / SDS

Discontinued Product

DYC789-2 has been discontinued. View all SMAC/Diablo products.

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell Lysates
Human Total SMAC/Diablo DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human Total SMAC/Diablo DuoSet IC ELISA

Summary for Human Total SMAC/Diablo DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total SMAC/Diablo DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total SMAC/Diablo DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SMAC/Diablo

SMAC (second mitochondria-derived activator of caspase)/Diablo, the mammalian functional homologue of Drosophila Hid, Grim, and Reaper, regulates apoptosis by neutralizing inhibitor of apoptosis (IAP) proteins. IAP family members contain one to three baculovirus IAP repeat (BIR) domains, each exhibiting distinct functions and caspase specificity. The BIR2 domain inhibits caspase-3 and –7, whereas the BIR3 domain inhibits caspase-9 activity. SMAC/Diablo interacts with BIR2 and BIR3 domains of IAPs, thereby neutralizing IAP inhibition and promoting caspase catalytic activity and apoptosis.

Item	Value
Long Name	Second Mitochondria-derived Activator of Caspase
Entrez Gene IDs	56616 (Human)
Alternate Names	0610041G12Rik, Diablo, Direct IAP-binding protein with low pI, SMAC, SMACmitochondrial, Second mitochondria-derived activator of caspase, diablo, IAP-binding mitochondrial protein, mitochondrial Smac protein

Citations for Human Total SMAC/Diablo DuoSet IC ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total SMAC/Diablo DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

Yan Y et al. (2004), Disturbed balance of expression between XIAP and Smac/DIABLO during tumour progression in renal cell carcinomas. Br. J. Cancer, 91(7):1349-57.
PMID: 15328523

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Product Documents for Human Total SMAC/Diablo DuoSet IC ELISA

Product Datasheets

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COA

SDS

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