Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTPA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL), Citrate Plasma (13 µL), Saliva (50 µL), Urine (25 µL), Human Milk (13 µL)
Sensitivity
16.1 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Saliva, Urine, Human Milk)
Product Summary for Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
The Quantikine Human t-Plasminogen Activator/tPA Immunoassay is a 4.5 hour solid phase ELISA designed to measure tPA levels in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human tPA and antibodies raised against the recombinant protein. Results obtained for naturally occurring human tPA showed linear curves that were parallel to the standard curves obtained using the Quantikine Human tPA Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human tPA.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human tPA. This assay also recognizes tPA complexed with PAI-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 498 | 1475 | 2878 | 490 | 1460 | 2898 |
| Standard Deviation | 36.0 | 48.4 | 88.8 | 32.1 | 62.3 | 115 |
| CV% | 7.2 | 3.3 | 3.1 | 6.6 | 4.3 | 4.0 |
Recovery for Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
The recovery of human tPA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 93 | 86-97 |
| Citrate Plasma (n=4) | 86 | 80-98 |
| EDTA Plasma (n=4) | 87 | 80-97 |
| Heparin Plasma (n=4) | 86 | 79-100 |
| Serum (n=4) | 95 | 87-109 |
| Urine (n=4) | 97 | 88-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human tPA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
Human t-Plasminogen Activator/tPA ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: t-Plasminogen Activator/tPA
tPA (tissue-type Plasminogen Activator) is a serine protease that is secreted by vascular endothelial cells, fibroblasts, neurons, microglia, and astrocytes. The partially active single chain can be further processed to full activity by Plasmin, Kallikrein 1, or Coagulation Factor Xa. Active tPA converts Plasminogen to Plasmin, a fibrinolytic protease, by hydrolyzing an Arg-Val peptide bond in Plasminogen. Unusually high levels of tPA activity can result in excessive bleeding, and low levels of tPA activity can result in thrombosis or embolism. tPA-mediated breakdown of the extracellular is also involved in promoting synaptic plasticity, neurite outgrowth, and synapse remodeling. Human tPA contains an N-terminal fibronectin type-1 domain, an epidermal growth factor-like domain, two kringle domains, and a serine protease catalytic domain.
Long Name
Tissue Plasminogen Activator
Alternate Names
Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator
Gene Symbol
PLAT
Additional t-Plasminogen Activator/tPA Products
Product Documents for Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
Product Specific Notices for Human t-Plasminogen Activator/tPA Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.