Skip to main content

Human SHBG Quantikine ELISA Kit

Bio-Techne includes R&D Systems | Catalog # DSHBG0B

Catalog #
Availability
Size / Price
Qty
Loading...
DSHBG0B

Control Products Available

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.012 nmol/L

Assay Range

0.063-4 nmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Product Summary for Human SHBG Quantikine ELISA Kit

The Quantikine® Human SHBG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SHBG in cell culture supernates, serum, and  plasma. It contains NS0-expressed recombinant human SHBG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SHBG showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SHBG.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human SHBG.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using one lot of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (nmol/L) 0.565 1.36 2.72 0.562 1.35 2.71
Standard Deviation 0.032 0.040 0.097 0.042 0.079 0.131
CV% 5.7 3.0 3.6 7.6 5.8 4.8

Recovery for Human SHBG Quantikine ELISA Kit

The recovery of human SHBG spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 87-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human SHBG were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human SHBG ELISA Linearity
Human SHBG Linearity

Scientific Data Images for Human SHBG Quantikine ELISA Kit

Human SHBG Standard Curve

Human SHBG Standard Curve

Human SHBG ELISA Standard Curve

Human SHBG ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SHBG

SHBG (Sex Hormone-binding Globulin), also known as ABP (Androgen-binding Protein), is a variably glycosylated, secreted, non-disulfide-linked homodimer. Male and female sex hormones bind SHBG with equal affinity. This binding protein can be found in the circulation where it regulates hormone access to target tissues. Human SHBG shares approximately 67% amino acid sequence identity with mouse and rat SHBG.

Long Name

Sex Hormone-binding Globulin

Alternate Names

ABP

Entrez Gene IDs

6462 (Human)

Gene Symbol

SHBG

Additional SHBG Products

Product Documents for Human SHBG Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SHBG Quantikine ELISA Kit

For research use only

Loading...
Loading...
Loading...
Loading...

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.