Human Proinsulin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPINS0
Discontinued Product
DPINS0 has been discontinued.
View all Proinsulin products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
1.43 pM
Assay Range
3.28-210 pM (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Proinsulin Quantikine ELISA Kit
The Quantikine Human Proinsulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Proinsulin in serum and plasma. It contains E.
coli-expressed recombinant human Proinsulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Proinsulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Proinsulin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Proinsulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pM) | 21.1 | 62.7 | 119 | 18.0 | 55.1 | 109 |
| Standard Deviation | 0.440 | 0.745 | 1.40 | 1.90 | 3.97 | 6.91 |
| CV% | 2.1 | 1.2 | 1.2 | 10.6 | 7.2 | 6.3 |
Recovery for Human Proinsulin Quantikine ELISA Kit
The recovery of human Proinsulin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 92 | 86-108 |
| Heparin Plasma (n=4) | 94 | 90-100 |
| Serum (n=4) | 95 | 80-105 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Proinsulin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Proinsulin Quantikine ELISA Kit
Human Proinsulin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Proinsulin
Insulin is a peptide hormone that is critical for glucose homeostasis. The mature Insulin peptide is derived from Proinsulin, which includes the Insulin A and B chains connected by a peptide fragment (Insulin C-peptide). Proinsulin is processed within the endoplasmic reticulum of pancreatic β cells into equimolar ratios of mature Insulin and Insulin C-peptide. Proinsulin binds the Insulin Receptor A isoform (IR-A) with high affinity resulting in cell proliferation and migration. It has low binding affinity for the Insulin Receptor B isoform (IR-B) and the Insulin-like Growth Factor I Receptor (IGF R1).
Alternate Names
IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin
Gene Symbol
INS
Additional Proinsulin Products
Product Documents for Human Proinsulin Quantikine ELISA Kit
Product Specific Notices for Human Proinsulin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.