Human Osteocalcin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSTCN0
Product is in the process of discontinuation. Please consider catalog # DY1419-05 as an alternative.
Discontinued Product
DSTCN0 has been discontinued.
View all Osteocalcin products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), EDTA Plasma (50 µL)
Sensitivity
0.898 ng/mL
Assay Range
2-64 ng/mL (Cell Culture Supernates, EDTA Plasma)
Product Summary for Human Osteocalcin Quantikine ELISA Kit
The Quantikine Human Osteocalcin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteocalcin in cell culture supernates and EDTA plasma. It contains synthetic human Osteocalcin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteocalcin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteocalcin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Osteocalcin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean (ng/mL) | 8.15 | 17.2 | 33.3 | 9.06 | 19.6 | 40.0 |
| Standard Deviation | 0.268 | 0.395 | 0.544 | 0.306 | 0.379 | 0.653 |
| CV% | 3.3 | 2.3 | 1.6 | 3.4 | 1.9 | 1.6 |
EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean (ng/mL) | 9.06 | 19.6 | 40.0 | 7.91 | 16.1 | 33.6 |
| Standard Deviation | 0.306 | 0.379 | 0.653 | 0.766 | 0.991 | 1.66 |
| CV% | 3.4 | 1.9 | 1.6 | 9.7 | 6.2 | 4.9 |
Recovery for Human Osteocalcin Quantikine ELISA Kit
The recovery of human Osteocalcin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 98-120 |
| EDTA Plasma (n=4) | 102 | 80-116 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Osteocalcin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Osteocalcin Quantikine ELISA Kit
Human Osteocalcin ELISA Cell Culture Supernate Standard Curve
Human Osteocalcin ELISA EDTA Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Osteocalcin
Long Name
Bone gamma-Carboxyglutamate [gla] Protein
Alternate Names
BGLAP, BGP, OCN
Gene Symbol
BGLAP
Additional Osteocalcin Products
Product Documents for Human Osteocalcin Quantikine ELISA Kit
Product Specific Notices for Human Osteocalcin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.