Human LC3A DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY8558-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
15.6-1000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human LC3A DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human LC3A DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Light Chain 3 (LC3). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human LC3A DuoSet ELISA
Human LC3A ELISA Standard Curve
Kit Contents for Human LC3A DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LC3A
The Autophagin protein family is involved in autophagy, the biological process in which endogenous protein and damaged organelles are degraded. ATG3 (autophagy-related protein 3; also known as APG3-like and PC3-96) is a ubiquitous 45 kDa member of the ATG3 family of proteins. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG7-bound ATG8 (also known as LC3, GATE16 and GABA-RAP in mammals) to phosphatidylethanolamine, critical for autophagy.
ATG4A (Autophagy related protein 4 homolog A; also known as Autophagin 2) is a 50 kDa cytosolic cysteine protease mostly found in skeletal muscle and brain. ATG10 (autophagy-related protein 10; also known as APG10-like) is a ubiquitous 28 kDa member of the ATG10 family of proteins. It serves as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG12 to ATG5. This ATG5:ATG12 heterodimer subsequently associates noncovalently with an ATG16 multimer to generate an autophagosome.
Long Name
Microtubule-associated Protein 1 Light Chain 3 alpha
Alternate Names
Apg8, APG8a, Apg8p3, ATG8E, LC3, MAP1ALC3, MAP1LC3A, MLP3A
Gene Symbol
MAP1LC3A
Additional LC3A Products
Product Documents for Human LC3A DuoSet ELISA
Product Specific Notices for Human LC3A DuoSet ELISA
For research use only
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