Human IL-10 QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK1064
Human IL-10 QuicKit ELISA
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
1.65 pg/mL
Assay Range
37.5-2400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-10 QuicKit ELISA
The Quantikine QuicKit Human IL-10 Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human IL-10 levels in cell culture supernates, serum, and plasma.It contains recombinant human IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-10.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 274 | 1317 | 272 | 1334 |
| Standard Deviation | 7.91 | 49.9 | 27.8 | 100 |
| CV% | 2.9 | 3.8 | 10.2 | 7.5 |
Recovery for Human IL-10 QuicKit ELISA
The recovery of IL-10 is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 96 | 87-105 |
| EDTA Plasma (n=2) | 84 | 75-90 |
| Heparin Plasma (n=2) | 78 | 65-97 |
| Serum (n=2) | 92 | 80-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-10 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-10 QuicKit ELISA
Human IL-10 Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-10
IL-10 (Interleukin-10) functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
Additional IL-10 Products
Product Documents for Human IL-10 QuicKit ELISA
Product Specific Notices for Human IL-10 QuicKit ELISA
For research use only
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