Human IL-10 QuantiGlo ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # Q1000
Discontinued Product
Q1000 has been discontinued.
View all IL-10 products.
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (40 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL)
Sensitivity
3.03 pg/mL
Assay Range
3.2-10000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-10 QuantiGlo ELISA Kit
The QuantiGlo Human IL-10 Chemiluminescent Immunoassay is a solid phase ELISA that specifically measures human IL-10 in less than 6 hours. It contains recombinant human IL-10 (expressed in Sf 21 insect cells using a baculovirus expression system) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-10 showed dose-response curves that are parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-10.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Chemiluminescent - Luminometer (Luminol)
Reactivity
Human
Specificity
Natural and recombinant human IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||||
|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 40 | 40 | 40 | 40 |
| Mean (pg/mL) | 25.9 | 67.1 | 578 | 5124 | 20.0 | 48.3 | 479 | 3945 |
| Standard Deviation | 0.83 | 1.50 | 26.0 | 204 | 3.87 | 4.30 | 23.3 | 222 |
| CV% | 3.2 | 2.2 | 4.5 | 4.0 | 19.4 | 8.9 | 4.9 | 5.6 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||||
|---|---|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 |
| n | 20 | 20 | 20 | 20 | 38 | 38 | 36 | 34 |
| Mean (pg/mL) | 17.3 | 57.8 | 597 | 5222 | 17.1 | 50.3 | 506 | 4188 |
| Standard Deviation | 0.85 | 1.85 | 26.3 | 67.9 | 3.35 | 5.40 | 32.0 | 276 |
| CV% | 4.9 | 3.2 | 4.4 | 1.3 | 19.6 | 10.7 | 6.3 | 6.6 |
Recovery for Human IL-10 QuantiGlo ELISA Kit
The recovery of natural and recombinant human IL-10 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 93-106 |
| EDTA Plasma (n=4) | 93 | 88-99 |
| Heparin Plasma (n=4) | 98 | 92-104 |
| Serum (n=4) | 97 | 90-103 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-10 were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-10 QuantiGlo ELISA Kit
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: IL-10
IL-10 (Interleukin-10) functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
Additional IL-10 Products
Product Documents for Human IL-10 QuantiGlo ELISA Kit
Product Specific Notices for Human IL-10 QuantiGlo ELISA Kit
For research use only
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