Human IGF-I/IGF-1 ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # DG100
Discontinued Product
DG100 has been discontinued.
An alternative/replacement product is available:
DG100B. View all IGF-I/IGF-1 products.
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity
0.056 ng/mL
Assay Range
0.094-6 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IGF-I/IGF-1 Quantikine ELISA Kit
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.490 | 1.243 | 2.373 | 0.382 | 0.967 | 1.941 |
| Standard Deviation | 0.017 | 0.054 | 0.103 | 0.031 | 0.080 | 0.145 |
| CV% | 3.5 | 4.3 | 4.3 | 8.1 | 8.3 | 7.5 |
Recovery for Human IGF-I/IGF-1 Quantikine ELISA Kit
The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 97-102 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IGF-I/IGF-1 Quantikine ELISA Kit
Human IGF-I ELISA Standard Curve
Human IGF-I/IGF-1 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I/IGF-1
IGF-1 (IGF-I, insulin-like growth factor-1 or somatomedin C) is a secreted factor that circulates in complex with IGFBP-3 or IGFBP-5 with ALS. It signals through dimeric IGF1R, dimeric Insulin R, or a heterodimer of the two receptors.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for Human IGF-I/IGF-1 Quantikine ELISA Kit
Product Specific Notices for Human IGF-I/IGF-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.