Human Free IGF-I/IGF-1 ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DFG100
Discontinued Product
DFG100 has been discontinued.
An alternative/replacement product is available:
DFG100B. View all IGF-I/IGF-1 products.
Key Product Details
Assay Length
1.75 hours
Sample Type & Volume Required Per Well
EDTA Plasma (50 µL)
Sensitivity
0.015 ng/mL
Assay Range
0.094-6 ng/mL (EDTA Plasma)
Product Summary for Human Free IGF-I/IGF-1 ELISA Kit
The Free IGF-I enzyme linked immunosorbent assay (ELISA) is a 1.75 hour solid-phase ELISA designed to measure human Free IGF-I in EDTA Plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Free IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 8 | 8 | 8 | 8 | 8 | 8 |
| Mean (pg/mL) | 0.20 | 0.83 | 1.87 | 0.18 | 0.80 | 2.09 |
| Standard Deviation | 0.01 | 0.03 | 0.07 | 0.02 | 0.08 | 0.13 |
| CV% | 5.0 | 3.6 | 3.7 | 11.1 | 10.0 | 6.2 |
Recovery for Human Free IGF-I/IGF-1 ELISA Kit
The recovery of samples spiked with IGF-I was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=3) | 107 | 93-118 |
Scientific Data Images for Human Free IGF-I/IGF-1 ELISA Kit
Human Free IGF-I Standard Curve
Human Free IGF-I ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I/IGF-1
Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.
The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for Human Free IGF-I/IGF-1 ELISA Kit
Product Specific Notices for Human Free IGF-I/IGF-1 ELISA Kit
For research use only
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