Human EPCR Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DEPCR0
Discontinued Product
DEPCR0 has been discontinued.
View all EPCR products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity
0.282 ng/mL
Assay Range
0.625-40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human EPCR Quantikine ELISA Kit
The Quantikine Human EPCR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human EPCR in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human EPCR and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human EPCR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring EPCR.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Reactivity
Human
Specificity
Natural and recombinant human EPCR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 5.2 | 11.7 | 21.5 | 5.26 | 10.7 | 21 |
| Standard Deviation | 0.2 | 0.6 | 1.7 | 0.3 | 0.7 | 1.3 |
| CV% | 4.8 | 4.9 | 7.7 | 5.4 | 6.4 | 6 |
Recovery for Human EPCR Quantikine ELISA Kit
The recovery of human EPCR spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=6) | 93 | 86-99 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of EPCR were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: EPCR
Long Name
Endothelial Protein C Receptor
Alternate Names
CCD41, CD201, PROCR
Gene Symbol
PROCR
Additional EPCR Products
Product Documents for Human EPCR Quantikine ELISA Kit
Product Specific Notices for Human EPCR Quantikine ELISA Kit
For research use only
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