Human Amyloid beta (aa1-40) ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DAB140B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Tissue Lysates (10 µL), Cerebrospinal Fluid (10 µL)
Sensitivity
8.17 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Product Summary for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
The Quantikine® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human Amyloid beta (aa1-40).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cerebrospinal Fluid, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 58.9 | 264 | 556 | 56.3 | 247 | 518 |
| Standard Deviation | 4.23 | 5.76 | 11.3 | 4.31 | 22.4 | 30.5 |
| CV% | 7.2 | 2.2 | 2.0 | 7.7 | 9.1 | 5.9 |
Recovery for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 95-105 |
| Cell Lysis Buffer (n=2) | 99 | 91-110 |
| Cerebrospinal Fluid (n=4) | 104 | 96-113 |
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
Human Amyloid beta (aa 1-40) ELISA Standard Curve
Human Amyloid beta (aa 1-40) ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Amyloid beta
Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.
Alternate Names
Abeta, beta-Amyloid Peptide
Gene Symbol
APP
Additional Amyloid beta Products
Product Documents for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
Product Specific Notices for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.