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Mouse CXCL10/IP-10/CRG-2 ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP1-92665

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NBP1-92665

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate), buffered solution (50 uL)

Sensitivity

6.5 pg/mL

Assay Range

7.8 - 500 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The assay detects both natural and recombinant mouse IP-10. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.40%

Inter-Assay Precision (Precision between assays) CV% < 7.20%

Recovery for Mouse CXCL10/IP-10/CRG-2 ELISA Kit (Colorimetric)

Recovery

The unspiked cell culture supernatant was used as blank in these experiments. The recovery ranged from 55% to 118% with an overall mean recovery of 90%.

Scientific Data Images for Mouse CXCL10/IP-10/CRG-2 ELISA Kit (Colorimetric)

Mouse CXCL10/IP-10/CRG-2 ELISA Kit [NBP1-92665] - Data for Mouse IP-10 Platinum ELISA

Kit Contents for Mouse CXCL10/IP-10/CRG-2 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse IP-10
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse IP-10 polyclonal antibody
  • Mouse IP-10 Standard lyophilized, 1000 pg/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CXCL10/IP-10/CRG-2

IP-10 is a member of the CXC subfamily of chemokines expressed by monocytes. It plays a pivotal role in immune system development and deployment. The Interferon-gamma inducible protein -10kD (IP-10) displays pleiotropic functions including stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Through this inhibitory effect on neovascularization IP-10 exhibits anti-tumoral effects. It shares a common receptor, CXCR3, with the chemokine MIG, but has been shown to play a distinct role in host defense in infections. IP-10 is further involved in allergic contact dermatitis reactions and other allergic diseases. It appears during immune-mediated processes, in inflammation of the nervous system and in Alzheimer's disease. Its involvement in the regulation of fibroproliferation following inflammatory lung injury has been described.

Alternate Names

CRG-2, CRG2, IP-10

Gene Symbol

CXCL10

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Mouse CXCL10/IP-10/CRG-2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse CXCL10/IP-10/CRG-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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