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Rat CTX-1 ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-69077

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NBP2-69077

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CTX1 in samples. No significant cross-reactivity or interference between Rat CTX1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.87%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Rat CTX-1 ELISA Kit (Colorimetric)

Recovery

92-110%

Linearity

Rat CTX-1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat CTX-1 ELISA Kit (Colorimetric)

Rat CTX-1 ELISA Kit (Colorimetric)

ELISA: Rat CTX-1 ELISA Kit (Colorimetric) [NBP2-69077] -

ELISA: Rat CTX-1 ELISA Kit (Colorimetric) [NBP2-69077] - Standard Curve Reference

Kit Contents for Rat CTX-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CTX-1

CTX-1, also referred to as either carboxy-terminal cross-linked telopeptide of type 1 collagen or C-terminal end of the telopeptide of type I collagen, is a marker of osteoporosis and bone resorption (1,2). CTX-1 is a common bone turnover marker (BTM) that can easily be measured in the plasma, serum, or urine (1-3). Bone turnover refers to the removal of old bones by resorption followed by generation of new bones (3). CTX-1 is released from intact collagen type 1 by cathepsin K cleavage and can be detected by an antibody against the eight amino acid sequence EKAHDGGR (2,4). Levels of CTX-1 show circadian rhythm variabilities altered by food intake, which makes timing of sample collection an important consideration and is typically done in a fasted state (1,3). Studies show that increased levels of CTX-1 along with bone-specific alkaline phosphatase (BSALP) is associated with increased risk for bone fractures and osteoporosis (3). The most common treatment for osteoporosis is bisphosphonates, which are classified as anti-resorptive drugs that slow the removal of bone (1,3). Another treatment is the Denosumab, a monoclonal antibody for receptor activator of NFkappaB ligand, which shows decreased levels of CTX-1 and other markers within the first 24-hours of treatment (1-3). Conversely, anabolic therapies add bone by focusing on the osteoclast and osteoblast populations. Romosozumab is one specific anabolic drug that showed quick decrease in CTX-1 levels following treatment (2,3).

References

1. Williams, C., & Sapra, A. (2020). Osteoporosis Markers. In StatPearls. StatPearls Publishing.

2. Szulc P. (2018). Bone turnover: Biology and assessment tools. Best Practice & Research. Clinical Endocrinology & Metabolism. https://doi.org/10.1016/j.beem.2018.05.003

3. Park, S. Y., Ahn, S. H., Yoo, J. I., Chung, Y. J., Jeon, Y. K., Yoon, B. H., Kim, H. Y., Lee, S. H., Lee, J., & Hong, S. (2019). Position Statement on the Use of Bone Turnover Markers for Osteoporosis Treatment. Journal of Bone Metabolism. https://doi.org/10.11005/jbm.2019.26.4.213

4. Cremers, C., Garnero, P., Seibel, M. J. (2008). Chapter 87- Biochemical Markers of Bone Metabolism. Principles of Bone Biology (Third Edition). https://doi.org/10.1016/B978-0-12-373884-4.00020-3.

Alternate Names

Collagen Type 1 C-Telopeptide, Cross Linked C-telopeptide of Type I Collagen, CTX, CTXI

Additional CTX-1 Products

Product Documents for Rat CTX-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CTX-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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