Human Cortisol - One Step ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23552

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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, urine, and saliva (50 uL)

Sensitivity

2.87 ng/mL

Assay Range

6.25-400 ng/mL

View all Cortisol ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Cortisol in samples. No significant cross-reactivity or interference between Human Cortisol and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 6.79

Inter-Assay Precision (Precision between assays) %CV < 5.64

Recovery for Human Cortisol - One Step ELISA Kit (Colorimetric)

Recovery

84-109%

Linearity

Scientific Data Images for Human Cortisol - One Step ELISA Kit (Colorimetric)

Human Cortisol ELISA Kit (Colorimetric)

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-23552] -

ELISA: Human Cortisol ELISA Kit (Colorimetric) [NBP3-23552] - Standard Curve Reference

Kit Contents for Human Cortisol - One Step ELISA Kit (Colorimetric)

  • Concentrated HRP Linked Detection Ab (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Linked Ab Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cortisol

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

Compound F, Hydrocortisone

Additional Cortisol Products

Product Documents for Human Cortisol - One Step ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Cortisol - One Step ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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