cGMP Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE003
Item in the process of being discontinued
Discontinued Product
KGE003 has been discontinued.
View all cGMP products.
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (100 µL), EDTA Plasma (100 µL), Saliva (100 µL), Urine (100 µL)
Sensitivity
3.06 pmol/mL
Assay Range
2.1-500 pmol/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Saliva, Urine)
Product Summary for cGMP Parameter Assay Kit
The Parameter cGMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cGMP in cell culture supernates, plasma, saliva, urine, and cell lysates.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures cyclic GMP levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 85.2 | 184 | 265 | 80.7 | 188 | 273 |
| Standard Deviation | 6.9 | 12.5 | 14.0 | 8.0 | 16.5 | 22.4 |
| CV% | 8.1 | 6.8 | 5.3 | 9.9 | 8.8 | 8.2 |
Cell Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 57.0 | 72.0 | 178 | 52.0 | 67.0 | 195 |
| Standard Deviation | 2.2 | 4.0 | 9.5 | 4.0 | 4.4 | 12.7 |
| CV% | 3.9 | 5.6 | 5.3 | 7.7 | 6.6 | 6.5 |
Recovery for cGMP Parameter Assay Kit
The recovery of cGMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 91-108 |
| Cell Lysates (n=1) | 92 | 80-98 |
| EDTA Plasma (n=4) | 91 | 74-105 |
| Saliva (n=4) | 102 | 80-123 |
| Urine (n=4) | 90 | 77-98 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cGMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for cGMP Parameter Assay Kit
Multi-species cGMP ELISA Cell Lysate Standard Curve
Multi-species cGMP ELISA Cell Culture Supernate/Plasma/Saliva/Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cGMP
Guanosine 3, 5-cyclic monophosphate (cGMP) is formed via the action of guanylate cyclase on GTP. cGMP is present at levels typically 10-100 fold lower than cAMP in most tissues. A variety of stimuli including acetylcholine, insulin, oxytocin, serotonin, and histamine will cause an increase in cGMP levels. Various stimulators of guanylate cyclase (e.g. vasodilators) as well as peptides that relax smooth muscle also increase cGMP concentrations.
Long Name
Cyclic GMP
Product Documents for cGMP Parameter Assay Kit
Product Specific Notices for cGMP Parameter Assay Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.